Regulatory

Part:BBa_K5211002:Design

Designed by: Niexin Zhou   Group: iGEM24_CHINA-HUBU-WUHAN   (2024-09-30)


The pGAP promoter is a strong constitutive promoter used for expressing foreign proteins in Pichia p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GTTCGATCAACAACCCGAATCCTATCGTAATGATGTTTTGCCCGATCAGCCTCAATCGACAATTTTACGCGTTTCGATCGAAGCAGGGACGACAATTGGCTGGGAACGGTATACTGGAATAAATGGTCTTCGTTATGGTATTGATGTTTTTGGTGCATCGGCCCCGGCGAATGATCTATATGCTCATTTCGGCTTGACCGCAGTCGGCATCACGAACAAGGTGTTGGCCGCGATCGCCGGTAAGTCGGCACGTTAAAAAATAGCTATGGAATATAGTAGCTACTTAATAAGTTAGGAGAATAAAC


Source

The pGAP promoter is a strong constitutive promoter used for expressing foreign proteins in Pichia pastoris. It originates from the GAPDH gene, which is stable and highly expressed in Pichia pastoris. It provides an effective tool for highly efficient expression of foreign proteins.

References